Analysis Note

Specific Activity: Human PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.

Application

For most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: E.coli

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.

Linkage

Replaces: GF017

Physical form

Lyophilized from a 10mM Acetic Acid solution.

Storage and Stability

The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in 10mM Acetic Acid to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.